Expressing:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Flow Cytometry:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Western Blot:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Over Expression:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Binding Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Gene Expression:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Activation Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Luciferase:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Reporter Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Positive Control:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Control:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Plasmid Preparation:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
RNA Sequencing:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Generated:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Transformation Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
MTT Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Standard Deviation:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Two Tailed Test:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Quantitative Proteomics:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Immunohistochemistry:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Knock-Out:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Modification:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Fluorescence:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Microscopy:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Clinical Proteomics:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Mass Spectrometry:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Centrifugation:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Purification:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Derivative Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Colony Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Transwell Migration Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Software:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Biomarker Discovery:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
In Vivo:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Injection:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Inhibition:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Mutagenesis:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Comparison:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Phospho-proteomics:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
shRNA:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Infection:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Transfection:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Ubiquitin Proteomics:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Isolation:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Transplantation Assay:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
Stable Transfection:Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.
Article Snippet: The human breast cancer cell lines MD A-MB-468, MD A-MB-231 were maintained in Dulbecco’ s modified Eagle’ s medium (DMEM) with 10% FBS and Penn / Strep, BT-549 was maintained in RPMI-1640 with 10% FBS and Penn / Strep, MCF-7 was maintained in Eagle’s minimal essential medium (EMEM) with 10% FBS and Penn / Strep and normal human breast cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep.cell line (hTERTHME-1) was maintained in DMEM F12 media with 10% FBS and Penn / Strep. ... The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was performed as described efore ( 30 ).MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, nner salt] assay for the second clone of eIF6-N106S homozyous knock-in HCT116 cells was..
Article Title: Role of Forkhead Box P3 in Interferon γ-mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554) and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.Mycoplasma testing was conducted upon cell line receipt using MycoProbe Mycoplasma Detection Kit (R&D Systems, Cat# CUL001B) and used within 6 months of resuscitation from frozen aliquots.
Article Title: Role of Forkhead Box P3 in IFNγ-Mediated PD-L1 Expression and Bladder Cancer Epithelial-to-Mesenchymal Transition
Article Snippet: Human bladder cancer cell lines HT1376 (RRID: CVCL_1292), T24 (RRID: CVCL_0554), and SW780 (RRID: CVCL_1728) were all originally authenticated from ATCC (karyotyping and morphology).. Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.Murine MB49 cell lines were originally derived from carcinogen-induced urothelial cell carcinomas in C57Bl6 mice, obtained from Timothy Ratliff.
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